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NF-κB Reporter (Luc) - CHO-K1 Recombinant Cell Line

●Purchase●DescriptionAnNF-BluciferasereporterconstructisstablyintegratedintothegenomeofCHO-K1cells

  • 產(chǎn)品型號:60622
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2023-11-11
  • 訪  問  量:181
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Description

An NF-κB luciferase reporter construct is stably integrated into the genome of CHO-K1 cells. The firefly luciferase gene is controlled by the NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

The NF-κB-luciferase / CHO-K1 cell line is suitable for monitoring the activity of NF-κB
transcription factor through luminescence readout.). This cell line responds to human cytokine
IL-1β, responds moderately to human TNF, and does not respond to human IFN-λ (2 μg/ml).
Reducing the amount of serum during incubation period may increase the sensitivity to
cytokines. Since CHO-K1 cells do not express endogenous human proteins, this cell line
provides an excellent platform to enable exogenous expression of a protein of interest to study
its downstream effect on NF-κB signaling.

This product has been cited 1 time.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line, nfkb, nf-kb
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