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GAS Reporter (Luc)-HeLa Cell Line (IFNγ/JAK/STAT1 Pathway)

  • 產(chǎn)品型號(hào):79041
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2023-11-11
  • 訪  問  量:180
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Description

The GAS reporter (Luc)-HeLa cell line is designed to monitor the activity of interferon gamma-induced signal transduction pathways in cultured cells by measuring activated STAT1 homodimers. It contains a firefly luciferase gene driven by three copies of the interferon gamma-activated sites (GAS) located upstream of the minimal TATA promoter. IFNγ first binds to a heterodimeric receptor consisting of two chains, IFNGR1 and IFNGR2, causing its dimerization and the activation of specific Janus family kinases (JAK1 and JAK2). Two STAT1 molecules associate with this ligand-activated receptor complex and are activated by phosphorylation to form active homodimer. The active STAT1 homodimers translocate to the nucleus where they bind interferon gamma-activated sites (GAS) in the promoter of IFNγ inducible genes, including luciferase reporter gene.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
Gas Cell line, GAS reporter cell line, GAS Hela cell line, STAT1, Just another kinase, JAK, INFγ, Signal transducer and activator of transcription 1, Interferon gamma cell line, JAK pathway bioassay
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