www亚洲午夜人美精片V区,国产精品乱码一区二区,亚洲另类黑,亚洲AV天天

熱門搜索: 50072ATAT1, GST-tag Recombinant 40520ATF2, GST-Tag Recombinant 31114GCN5 (727-837), His-tag Recombinant 50074GCN5 (KAT2A), FLAG-tag Recombinant 50071p300 (KAT3B), His-GST-tag Recombinant 11004ACHE, His-Tag Recombinant 50272BCL-2, His-Tag Recombinant 100077BCL2A1, His-Tag Recombinant 100080BCL2L10, His-Tag Recombinant 100091BCL2L2, His-Tag Recombinant 80113Caspase6, His-tag Recombinant 70000Caspase7, His-tag Recombinant 80114Caspase8, His-tag Recombinant 100543GPX4, FLAG-Tag Recombinant 100054MALT1, FLAG-Tag (Mouse) Recombinant 100637MCL-1 His-Tag (Mouse) Recombinant

產(chǎn)品展示/ Product display

您的位置:首頁  /  產(chǎn)品展示  /    /  Ras Signaling Assay Kits  /  78519KRAS(G12V) Nucleotide Exchange Assay Kit

KRAS(G12V) Nucleotide Exchange Assay Kit

  • 產(chǎn)品型號:78519
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2023-11-11
  • 訪  問  量:227
立即咨詢

聯(lián)系電話:

詳細介紹
Purchase
Description

The KRAS(G12V) Nucleotide Exchange Assay is a homogeneous assay designed for the screening and profiling of KRAS(G12V) antagonists/inhibitors by using BODIPY®-GDP to monitor the GDP or GTP binding status. The kit can be used with two different protocols for greater flexibility, either titrating the inhibitor at a fixed GTP concentration or titrating the GTP at a fixed inhibitor concentration.

BODIPY® FL-GDP is a mixed isomer in which the fluorophore has been attached to the 2’ or 3’ position of the ribose ring via a linker. It is a green-fluorescent dye with similar excitation and emission to fluorescein or Alexa Fluor™ 488, characterized by a high extinction coefficient and high quantum yield and is relatively insensitive to pH changes. The dye has an excited-state lifetime of 5 nanoseconds or longer.

Assay Principle Illustration: KRAS is activated upon binding GTP, when it undergoes a conformational change. KRAS then returns to a GDP-bound inactive state following the hydrolysis of GTP to GDP. In this assay, KRAS is pre-loaded with fluorescent BODIPY®-GDP and therefore is inactive. Adding GTP in the presence of EDTA displaces BODIPY®-GDP because KRAS affinity for GTP is greater than its affinity for GDP. The fluorescence intensity decreases as the BODIPY®-GDP is displaced. Several KRAS inhibitors lock KRAS in the (inactive) GDP-bound conformation and prevent GDP/GTP exchange. In this scenario the fluorescence intensity increases with drug concentration as more BODIPY®-GDP remains bound to KRAS.

Synonyms
GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed, KRAS2, RASK2
留言詢價

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
關(guān)注公眾號

郵箱:infocn@bpsbioscience.com

傳真:

地址:上海靜安區(qū)

Copyright © 2025 BPS Bioscience Inc.版權(quán)所有   備案號:   技術(shù)支持:化工儀器網(wǎng)

TEL:

掃碼加微信