The PDE4A Assay Kit is designed for identification of PDE4A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4A to the binding agent. The key to the PDE4A1A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4A1A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4A1A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

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