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Fluoro-Verse™ ACE2 Inhibitor Assay Kit

  • 產(chǎn)品型號(hào):78847
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2023-11-12
  • 訪  問  量:151
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Description

The Fluoro-Verse™ Angiotensin Converting Enzyme 2 (ACE2) Inhibitor Assay Kit is a 96-well homogeneous fluorogenic assay designed to measure the exopeptidase activity of ACE2 for screening and profiling applications. The ACE2 assay kit contains enough purified ACE2 (BPS Bioscience #11003, amino acids 18-740), its substrate, and ACE2 buffer for 100 reactions.

Figure 1: Illustration of the principle behind the ACE2 fluorogenic assay.
The assay uses an ACE2 substrate in which the fluorescence emitted by Mca (7-Methoxycoumarin-4-acetic acid) is quenched by the proximal Dnp (2,4-dinitrophenyl) quencher. ACE2 is a carboxypeptidase that cleaves the C-terminus peptide bond. Thus, Mca is released from quenching upon proteolysis and emits fluorescence with excitation/emission maxima of 320/380 nm. The increase in fluorescence is proportional to ACE2 activity.

Synonyms
ACE2, ACEH, angiotensin I converting enzyme 2, sars, sars protease
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