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PCSK9(His)-LDLR Binding Assay Kit

●Purchase●DescriptionThePCSK9(His)-LDLRBindingAssayKitisanELISA-type96-wellformatassaydesignedforscreeningandprofilingpurposes

  • 產(chǎn)品型號:78813
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2023-11-12
  • 訪  問  量:247
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Description

The PCSK9(His)-LDLR Binding Assay Kit is an ELISA-type 96-well format assay designed for screening and profiling purposes. Moreover, two pre-formulated assay buffers are supplied to validate PCSK9-LDLR binding affinity in either neutral or acidic binding conditions. The assay takes advantage of the high sensitivity of detection of His-tag PCSK9 by Anti-His HRP-antibody. First, the LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with Anti-His HRP-antibody followed by addition of an HRP substrate to produce chemiluminescence, measured using a chemiluminescence reader.

Figure 1: Illustration of the ELISA-based PCSK9(His)-LDLR Binding Assay Kit.

Synonyms
Low-density lipoprotein receptor, LDL receptor, Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1, NARC-1, Proprotein convertase 9, PC9, Subtilisin/kexin-like protease PC9, NARC1, PSEC0052
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