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WWP1 Intrachain TR-FRET Assay Kit

●Purchase●DescriptionTheWWP1intrachainTR-FRETAssayKitutilizesaEuropiumcryptate-labeledUbiquitin(donor)aswellasaCy5-labeledUbiquitin(acceptor)tocompletetheTR-FRETpairing

  • 產(chǎn)品型號：78554
  • 廠商性質(zhì)：生產(chǎn)廠家
  • 更新時(shí)間：2023-11-12
  • 訪  問  量：185
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Description

The WWP1 intrachain TR-FRET Assay Kit utilizes a Europium cryptate-labeled Ubiquitin (donor) as well as a Cy5-labeled Ubiquitin (acceptor) to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on WWP1, this FRET-based assay requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time kinetic analyses of polyubiquitination.

Figure 1. WWP1 intrachain TR-FRET Assay Kit schematic

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