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NF-κB Reporter Cellular Assay Pack (HCT116)

●Purchase●DescriptionTheNF-κBReporterCellularAssayPackprovidesallthekeyreagentsrequiredtomonitortheactivityofthenuclearfactorKappaB(NF-κB)signaltransductionpathways

  • 產(chǎn)品型號:79326
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2023-11-11
  • 訪  問  量:150
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Description

The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc) - HCT-116 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine.

Additionally, the pack includes cell culture medium (Thaw Medium 7) that has been optimized for use with HCT-116 cells. Thaw Medium 7 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Synonyms
NF-κB Cell Line, NFkappaB Cell Line, Luciferase Reporter, NF-kB Cell Line, NFkB Cell Line, nfkb, nf-kb
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